This AACR Journal Club discusses features Peter Sorger, PhD, discussing his article “The Spatial Landscape of Progression and Immunoediting in Primary Melanoma at Single-Cell Resolution.” The intricacies of primary melanoma progression are revealed by a combination of high-plex imaging, 3D high-resolution microscopy, and spatially resolved microregion transcriptomics. Dr. Sorger shows that this unprecedented spatiotemoporal resolution uncovers recurrent cellular neighborhoods and the landscape of immune evasion and immunoediting.

This webinar presents two projects from the Ludwig Centers at Harvard and MIT that are part of the Ludwig Tumor Atlas Project. The Ludwig Tumor Atlas applies diverse technologies to probe the tumor environment using a variety of methods, including highly multiplexed tissue imaging, dissociative single cell sequencing, spatial transcriptomics and conventional histology.

Zoltan Maliga, PhD, Scientific Director of Tissue Imaging at the Laboratory of Systems Pharmacology, describes using multiplexed fluorescent tissue imaging to characterize Formalin-Fixed Paraffin-Embedded (FFPE) tissues.

Bree Aldridge, PhD, Associate Professor at Tufts University School of Medicine and Tufts University School of Engineering, introduces the Tuberculosis Tumor Atlas.

Ben Izar, MD, PhD, Medical Oncologist at Dana-Farber Cancer Institute, talks about how multiplexed immunofluorescence imaging using t-CyCIF is revealing the architecture of tumors and the many types of cells involved.